Bioautography in fish—II.

Bioautography detection in thin-layer chromatography.

Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of ...

[Tetrazolium bioautography of pteroylglutamic acid conjugates].

A microbioautographic method for the identification of pteroylglutamic acid derivatives and their glutamyl conjugates is described. Chromatograms or pherograms of mixtures of the compounds are treated with chicken pancreas conjugase, followed by tetrazolium bioautography. This combined procedure allows qualitative and semi-quantitative analyses of folate compounds in biological material in the ...

Antibacterial activities of Dodonaea viscosa using contact bioautography technique.

The crude ethanolic extract and n-hexane, dichloromethane, ethyl acetate, n-butanol and aqueous fractions of Dodonaea viscosa were analyzed for antibacterial potential against four Gram positive bacteria: Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, and three Gram negative bacteria: Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa. Preliminary screeni...

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

The richness of bioactive compounds in plant materials encourages continuous development of separation methods and bioassays for their isolation and identification. Thin-layer chromatography-direct bioautography links separation on the adsorbent layer with biological tests performed directly on it. Therefore, the method is very convenient for searching plant constituents with biological activit...

Analysis of quaternary ammonium compounds and hexachlorophene by thin-layer chromatography and agar diffusion bioautography.